Antibacterial and phytochemical analysis of stem and root extracts of Calotropis gigantea against selected pathogens

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Authors :

Kalaivani Radhakrishnan1, Panneerselvam Thangamani1 and Vedha Balakrishnan1*

Author Address :

1Department of Microbiology, Adhiparasakthi College of Arts & Science, G.B. Nagar, Kalavai- 632 506. * For correspondence:bvedha1980@rediffmail.com

Abstract :

The extracts of Caliotropis gigantea (stem and root extracts) were tested against clinical isolates of E. coli, Pseudomonas aeruginosa, Salmonella typhi, Vibrio chloerae and Staphylococcus aureus. The agar well diffusion assay method was used to access the activities of plant extract against the isolated organisms. All the extracts of stem and root showed good antimicrobial activity against the isolated organisms. The ethanol extract of stem are more effective to the organism. The methanol extract of root are more effective to the organism. The phytochemical analysis can be carried out on the ethanol extract of stem and methanol extract of root shows the presence of quinones, steroids, terpenoids, thin layer chromatography (TLC) have been carried out on ethanol extract (stem), methanol extract (root) of Caliotropis gigantea, which show different Rf values and possible combinations of chromatography solvents for separation of these phytochemicals.

Keywords :

Caliotropis gigantea, medicinal plants, Antibacterial activity, phytochemicals, thin layer chromatography

Article Info :

Received 07-Nov-2013, Revised 03-Apr-2014, Accepted 12-Dec-2013

 

 

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