Virulence factors and multidrug resistance of uropathogenic Escherichia Coli isolated from men with urinary tract infection in and around Coimbatore, South India

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Authors :

 Vijayanand R and Mani K R*

Author Address :

1Department of Microbiology and Bioinformatics, CMS College of Science and Commerce, Chinnavedampatti, Coimbatore, Tamilnadu, India-641049. 2Central Research Institute, (Kasauli, Himachal Pradesh, India), Pasteur Institute of India, Coonoor, Tamilnadu, India-643103.
*For correspondence E-mail: vijayanandramakrishnan@yahoo.co.in

Abstract :

Urinary tract infection (UTI) has become prominent disease caused predominantly by uropathogenic Escherichia coli (UPEC) and other members of Enterobacteriaceae family. The bacteria will enter the urethra and infect bladder (cystitis), kidney (pyelonephritis) and blood stream (septicemia). The virulence factors and serotypes influence the pathogenicity. Unwanted usage of low standard antibiotics leads to multidrug resistance of the bacteria. The UPEC produces extended spectrum beta lactamase (ESBL) in order to degrade certain drugs. The prevalence of UTI is more in women when compared to men due to urogenetial anatomy. Therefore, treatment till date given is based on the data from women patients. Since the UPEC isolated from men behaved differently in their sensitivity pattern, they must be treated separately. In this way we have isolated 100 UPEC from men attending hospitals in and around Coimbatore, South India and analyzed. No cases were identified in adult between 21 and 30 years, above 60 years more cases were reported. The highest expressed virulence factor was hemolysis (64%) followed by serum resistance (40%) and MSHA (36%). Most of them (72%) were ESBL producers and the antibiotic sensitivity pattern revealed that the isolates were highly resistant to ampicillin (84%), cephazolin and cefepime (72%). The least resistant was against imipenem and meropenem (4%). Commonly encountered serotypes were rough, O8, O9 and O2. To conclude, men of age above 60 years were vulnerable to UTI by UPEC which are hemolytic. Above 70% were ESBL producers and highly resistant to ampicillin, cephazolin and cefepime. They were sensitive to imipenem and meropenem towards which resistance started developing. Common serotypes of UTI were absent and random serotypes were detected. This work may help treating UTI in men and research in this field is essential to understand and treat UTI in men.

Keywords :

Uropathogenic, Escherichia coli, Virulence factor, Multidrug Resistant, Serotype.

DOI :

Article Info :

Received 02-Feb-2018, Revised 16-Apr-2018, Accepted 10-Apr-2018

 

 

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