Removal of Chromium from contaminated effluent and simultaneously Green Nanoparticle synthesis using Bacillus subtilis

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Authors :

Kanakalakshmi Annamalai1, Anjali Mohan nair2, Sukumar Chinnaraju2 and Shanthi Kuppusamy1*

Author Address :

1Department of Environmental Science, PSG College of Arts and Science, Coimbatore-641014, Tamil Nadu, India
2Department of Environmental Science, PSG College of Arts and Science, Coimbatore-641014, Tamil Nadu, India
* For correspondence e-mail:

Abstract :

The wide spread industrial application of chromium leads to the dangerous effects to the environment. In recent years the removal of heavy metals from the industrial effluents using microorganisms is becoming a novel approach in recent years. Bacillus species exhibited the property of biosorption of Cr (VI) to Cr (III) and it simultaneously converted the Cr into nanoparticle. The Cr removal studies were carried out using different concentration of potassium dichromate solution (50,100,150,200 and 250ppm) in the growth media, using various pH ranges (2, 3, 5, 7 and 9) and biomass at different dosage (1-5ml) under 37°C. The batch experiment was carried out with a initial Cr concentration (100 mg/l), pH 7 and 1ml of biomass dosage. The maximum percentage of removal was found to be 99% and along with it nanoparticle was also synthesized. The nanoparticles synthesised were characterised using UV-Visible Spectrophotometer, Fourier Transmission Infra-Red Spectrophotometer (FTIR), X-ray Diffraction (XRD) and Scanning electron microscope (SEM). Thus the study proves that the strain would completely remove chromium from the aqueous solution and it provides a facile green technology for the synthesis of nanoparticle.

Keywords :

Chromium, Bacillus subtilis, Nanoparticle, Green Technology, FTIR, XRD.


Article Info :

Received 10-Nov-2013, Revised 03-Apr-2014, Accepted 02-Dec-2013



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