Changes in antioxidative enzymes and isozyme pattern due to Exobasidium vexans (blister blight) infection in tea cultivars

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Authors :

Shalini D1* and Raj kumar R2

Author Address :

1SNS College of Engineering, Coimbatore-641017.
2Department of Plant Physiology and Biotechnology, UPASI Tea Research Foundation, Tea Research Institute, Valparai, Coimbatore-642127.
*For correspondence:

Abstract :

Assessment of antioxidative enzymes and their isoenzyme pattern were studied with field grown tea plants having highly resistant (SA-6 & SMP-1) and highly susceptible (UPASI-14 & TES 31) traits to Exobasidium vexans. Antioxidative enzymes were assayed in healthy and infected leaves collected from selected tea clones. Peroxidase, ascorbate peroxidase and glutathione reductase have higher activity in resistant clones but catalase activity was very low while there was no trend in SOD activity. The zymograms of peroxidase and catalase in the resistant genotype showed amplified expression than susceptible tea clones. Induction and /or amplified expression of particular isoforms of peroxidase and catalase against blister blight infection in resistant tea clones led to the apparent conclusion that amplified isoenzyme expression linked with blight resistance. This might be considered as an important criterion in breeding programme to identify the Exobasidium resistant genotype in tea.

Keywords :

Isoenzyme, antioxidative enzymes, blister blight

Article Info :

Received 18-Feb-2017, Revised 27-Apr-2017, Accepted 16-May-2017



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