Isolation and characterization of Indole acetic acid producing strain from rhizospheric forest soil of Visakhapatnam District, Andhra Pradesh, India

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Authors :

Devi Soundarya S and Padal S B

Author Address :

Department of Botany, Andhra University, Visakhapatnam - 530003, A.P, India.
For Correspondence E-mail: devisoundarya32@gmail.com

Abstract :

Plant growth promoting rhizobacteria (PGPR) are of great importance in agriculture for better crop yield without pathological stress to plants and soil as well. Indole Acetic Acid (IAA), one of the plant growth promoting components, is an important property of rhizosphere bacteria which facilitates adequate crop yield. It is already reported that IAA producing bacteria is an efficient biofertilizer in agriculture to promote plant growth. Hence, isolation of such bacteria is very crucial for agriculture fields for better nutrition. The present study is aimed to isolate IAA producing bacteria from different forest soils (Salugu, Vanthalamamidi, Panneddu mailu, Dallapalle and Gurragaruvu village) of Paderu mandal, Vishakhapatnam district, India. Organisms isolated from these rhizosphere soils were characterized and screened for IAA production. Among the 55 isolated bacteria, 6 isolates (from Gurragaruvu village) showed IAA producing activity and out of these 6 isolates one isolate showed high IAA production. Then 16S rRNA gene sequencing was performed for this promising isolate and based on the sequence homology, the bacterial isolate is identified as Klebsiella Pneumonia.

Keywords :

Klebsiella pneumonia, IAA, PGPR, In vitro and 16S rRNA

DOI :

Article Info :

Received 27-Nov-2017, Revised 26-Dec-2017, Accepted 28-Dec-2017

 

 

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